Functional Genomics

Edited by Kaufmann, Michael; Edited by Klinger, Claudia

Over the last decade Life Science has undergone an accelerated evolution, culminating in the -omics era characterized by the development of a multitude of high throughput methods that are becoming more routinely applied in biochemistry labs. In Functional Genomics: Methods and Protocols, Second Edition expert researchers in the field detail many of the methods which are now commonly used for studies in the life sciences focusing on the dynamic aspects of the transcriptome, proteome and metabolome, respectively.Written in the highly successful Methods in Molecular Biology(t) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Functional Genomics: Methods and Protocols, Second Edition seeks to aid scientists in establishing or extending technologies and techniques in their laboratories.

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[目次]

  • Part I: Bioinformatics 1. Prediction of Protein Tertiary Structures using MUFOLD Jingfen Zhang, Zhiquan He, Qingguo Wang, Bogdan Barz, Ioan Kosztin, Yi Shang, and Dong Xu 2. Prediction of Protein Functions Roy D. Sleator 3. Genome Wide Screens for Expressed Hypothetical Proteins Claus Desler, Jon Ambaek Durhuus and Lene Juel Rasmussen 4. Self Custom-Made SFP Arrays for Non-Model Organisms Ron Ophir and Amir Sherman Part II: DNA Analysis 5. Construction and Analysis of Full-length and Normalized cDNA Libraries from Citrus M Carmen Marques and Miguel A Perez-Amador 6. Assembling Linear DNA Templates for In vitro Transcription and Translation Viktor Stein, Miriam Kaltenbach and Florian Hollfelder 7. Automated Computational Analysis of Genome-wide DNA Methylation Profiling Data from HELP-tagging Assays Qiang Jing, Andrew McLellan, John M. Greally and Masako Suzuki Part III: RNA Analysis 8. Detecting of RNA Editing Events in Human Cells using High-Throughput Sequencing Iouri Chepelev 9. Comparative Study of Differential Gene Expression in Closely Related Bacterial Species by Comparative Hybridization Ruisheng An and Parwinder S. Grewal 10. Whole Genome RT-qPCR MicroRNA Expression Profiling Pieter Mestdagh, Stefaan Derveaux, Jo Vandesompele 11. Using Quantitative Real-Time Reverse Transcriptase Polymerase Chain Reaction to Validate Gene Regulation by PTTG Siva Kumar Panguluri and Sham S. Kakar 12. FRET-Based Real-Time DNA Microarrays Arjang Hassibi, Haris Vikalo, Jose Riechmann and Babak Hassibi Part IV: Protein Analysis I: Quantification and Indentification 13. 2-D Gel Electrophoresis: Constructing 2D-Gel Proteome Reference Maps Maria Paola Simula, Agata Notarpietro, Giuseppe Toffoli, and Valli De Re 14. The Use of Antigen Microarrays in Antibody Profiling Krisztian Papp and Jozsef Prechl 15. Limited Proteolysis in Proteomics using Protease-immobilized Microreactors Hiroshi Yamaguchi, Masaya Miyazaki and Hideaki Maeda 16. Mass Spectrometry for Protein Quantification in Biomarker Discovery Mu Wang and Jinsam You Part V: Protein Analysis II: Functional Characterization 17. High-throughput Microtitre Plate-based Assay for DNA Topoisomerases James A Taylor, Nicolas P Burton and Anthony Maxwell 18. Microscale Thermophoresis as a Sensitive Method to Quantify Protein-Nucleic Acid Interactions in Solutions Karina Zillner, Moran Jerabek-Willemsen, Stefan Duhr, Dieter Braun, Gernot Langst and Philipp Baaske 19 Bioluminescence Resonance Energy Transfer: an Emerging Tool for the Detection of Protein-protein Interaction in Living Cells Soren W. Gersting, Amelie S. Lotz-Havla and Ania C. Muntau 20 LuMPIS - Luciferase-based MBP-pull-down Protein Interaction Screening System Maria G. Vizoso Pinto and Armin Baiker 21 Yeast Two-Hybrid Screens: Improvement of Array-based Screening Results by N- and C-terminally Tagged Fusion Proteins Thorsten Stellberger, Roman Hauser, Peter Uetz, and Albrecht von Brunn 22. Inducible microRNA-mediated Knockdown of the Endogenous Human Lamin A/C Gene Ina Weidenfeld 23. Multiple-gene Silencing using Antisense RNAs in Escherichia Coli Nobutaka Nakashima, Shan Goh, Liam Good and Tomohiro Tamura 24. Functional Screen of Zebrafish Deubiquitylating Enzymes by Morpholino Knockdown and in Situ Hybridization William Ka Fai Tse and Yun-Jin Jiang 25. Silencing of Gene Expression by Gymnotic Delivery of Antisense Oligonucleotides Harris S. Soifer, Troels Koch, Johnathan Lai, Bo Hansen, Anja Hoeg, Henrik Oerum and C.A. Stein 26. Polycistronic Expression of Interfering RNAs and RNA Plymerase III Promoters Laura F. Steel and Viraj R. Sanghvi Part VI: Metabolite Analysis 27. Metabolite Analysis of Cannabis sativa L. by NMR Spectroscopy Isvett Josefina Flores-Sanchez, Young Hae Choi and Robert Verpoorte 28. Metabolome Analysis of Grame-positive Bacteria such as Staphylococcus aureus by GC-MS and LC MS Manuel Liebeke, Kirsten Dorries, Hanna Meyer and Micheal Lalk 29. Metabolic Fingerprinting Using Comprehensive Two-dimensional Gas Chromatography-Time-of-Flight Mass Spectrometry(GCxGX-TOF-MS) Martin F. Almstetter, Peter J. Oefner, and Katja Dettmer

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この本の情報

書名 Functional Genomics
著作者等 Klinger, Claudia
Kaufmann, Michael
シリーズ名 Methods in Molecular Biology 815
出版元 Humana Press Inc.
刊行年月 2011.11.18
版表示 2nd ed. 2012
ページ数 377p
大きさ H254 x W178
ISBN 9781617794230
言語 英語
出版国 アメリカ合衆国
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