Principles of fluorescence spectroscopy

Joseph R. Lakowicz

The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.

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[目次]

  • Introduction to Fluorescence.- Instrumentation for Fluorecence Spectroscopy.- Fluorophores.- Time-Domain Lifetime Measurements.- Frequency-Domain Lifetime Measurements.- Solvent and Environmental Effects.- Dynamics of Solvent and Spectral Relaxation.- Quenching of Fluorescence.- Mechanisms and Dynamics of Fluorescence Quenching.- Fluorescence Anisotropy.- Time-Dependent Anisotropy Decays.- Advanced Anisotropy Concepts.- Energy Transfer.- Time Resolved Energy Transfer and Conformational Distributions of Biopolymers.- Energy Transfer to Multiple Acceptors, in One-, Two- or Three-Dimensions.- Protein Fluorescence.- Time-Resolved Protein Fluorescence.- Multiphoton Excitation and Microscopy.- Fluorescence Sensing.- Novel Fluorophobes.- DNA Technology.- Fluorescence Lifetime Imaging Microscopy.- Single-Molecule Detection.- Fluorescence Correlation Spectroscopy.- Radiative Decay Engineering: Metal-Enhanced Fluorescence.- Radiative Decay Engineering: Surface Plasmon-Coupled Emission.- Appendix I. Corrected Emission Spectra.- Appendix II. Fluorescent Lifetime Standards.- Appendix III. Additional Reading.- Answers to Problems.- Index.

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この本の情報

書名 Principles of fluorescence spectroscopy
著作者等 Lakowicz, Joseph R.
出版元 Springer
刊行年月 c2006
版表示 3rd ed
ページ数 xxvi, 954 p.
大きさ 29 cm.
付随資料 1 CD-ROM
ISBN 0387312781
9781489978806
NCID BA78210628
※クリックでCiNii Booksを表示
言語 英語
出版国 アメリカ合衆国
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