Yeast protocols

edited by Wei Xiao

In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research and for using yeast as a host to study genes from other organisms. The authors provide detailed methods for the isolation of subcellular components-including organelles and macromolecules, for the basic cellular and molecular analysis specific for yeast cells, and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells.

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[目次]

  • Basic Investigations in Saccharomyces cerevisiae Brendan P. G. Curran and Virginia Bugeja Isolation of Nucleic Acids Michelle Hanna and Wei Xiao Purification of Yeast Peroxisomes Ben Distel and Astrid Kragt Isolation of Yeast Plasma Membranes Barry Panaretou and Peter Piper Isolation of Yeast Mitochondria Chris Meisinger, Nikolaus Pfanner, and Kaye N. Truscott Extraction of Yeast Lipids Roger Schneiter and Gunther Daum Two-Dimensional Gel Electrophoresis of Total Yeast Proteins Helian Boucherie and Christelle Monribot-Espagne Pulsed-Field Gel Electrophoresis of Budding Yeast Chromosomes Laura Maringele and David Lydall. Analysis of Yeast Lipids, Roger Schneiter and Gunther Daum Yeast Fluorescence Microscopy Jiri Hasek Intracellular Expression of Recombinant Antibody Fluorescent Protein Fusions for Localization of Target Antigens in Schizosaccharomyces pombe Michelle A. Alting-Mees, Eddy P. Risseeuw, Enwu Liu, Michel Desautels, William A. Crosby, and Sean M. Hemmingsen Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method R. Daniel Gietz and Robin A. Woods Mutagenesis Leslie Barbour, Michelle Hanna, and Wei Xiao Gene Disruption in the Budding Yeast Saccharomyces cerevisiae Johannes H. Hegemann, Ulrich Guldener, and Gabriele J. Kohler Inducible Degron and Its Application to Creating Conditional Mutants R. Jurgen Dohmen Synthetic Lethal Screen Leslie Barbour and Wei Xiao Synthetic Genetic Array Analysis in Saccharomyces cerevisiae Amy Hin Yan Tong and Charles Boone Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates Alain T. Dandjinou, Michel Larrivee, Ralf E. Wellinger, and Raymund J. Wellinger Chromatin Assembly in a Crude Fraction From Yeast Cells Karen M.Robinson and Michael C. Schultz Chromatin Immunoprecipitation to Study Protein-DNA Interactions in Budding Yeast Elena Ezhkova and William P. Tansey Isolation of Yeast Nuclei and Micrococcal Nuclease Mapping of Nucleosome Positioning Zhengjian Zhang and Joseph C. Reese Study of Transcriptional Regulation Using a Reporter Gene Assay Yu Fu and Wei Xiao Assessing Telomeric Phenotypes Catherine LeBel, Michel Larrivee, Amadou Bah, Nancy Laterreur, Nancy Levesque, and Raymund J. Wellinger Controlled Expression of Recombinant Genes and Preparation of Cell-Free Extracts in Yeast Zhigang Wang Production of Heterologous Proteins in Yeast With the Aid of the Hsp150D Carrier Marja Makarow, Anna-Liisa Hanninen, Taina Suntio, and Ricardo Nunes Bastos Yeast Two-Hybrid System Screening R. Daniel Gietz Import of Precursor Proteins Into Isolated Yeast Mitochondria Nils Wiedemann, Nikolaus Pfanner, and Peter Rehling Index

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この本の情報

書名 Yeast protocols
著作者等 Wei Xiao
Xiao Wei
シリーズ名 Methods in molecular biology
出版元 Humana Press
刊行年月 c2006
版表示 2nd ed.
ページ数 xi, 392 p.
大きさ 24 cm.
ISBN 9781617375699
NCID BA7432963X
※クリックでCiNii Booksを表示
言語 英語
出版国 アメリカ合衆国

掲載作品

著作名 著作者名
Analysis of yeast lipids Günther Daum, Roger Schneiter
Assessing telomeric phenotypes Catherine LeBel
Basic investigations in saccharomyces cerevisiae Brendan P.G. Curran, Virginia Bugeja
Chromatin assembly in a crude fraction from yeast cells Karen M. Robinson, Michael C. Schultz
Chromatin immunoprecipitation to study protein-DNA interactions in budding yeast Elena Ezhkova, William P. Tansey
Controlled expression of recombinant genes and preparation of cell-free extracts in yeast Zhigang Wang
Extraction of yeast lipids Günther Daum, Roger Schneiter
Gene disruption in the budding yeast saccharomyces cerevisiae Gabriele J. Köhler, Johannes H. Hegemann, Ulrich Güldener
Import of precursor proteins into isolated yeast mitochondria Nikolaus Pfanner, Nils Wiedemann, Peter Rehling
Inducible degron and its application to creating conditional mutants R. Jürgen Dohmen
Intracellular expression of recombinant antibody fluorescent protein fusions for localization of target antigens in schizosaccharomyces pombe Michelle A. Alting-Mees
Isolation of nucleic acids Michelle Hanna, Wei Xiao
Isolation of yeast mitochondria Chris Meisinger, Kaye N. Truscott, Nikolaus Pfanner
Isolation of yeast nuclei and micrococcal nuclease mapping of nucleosome positioning Joseph C. Reese, Zhengjian Zhang
Isolation of yeast plasma membranes Barry Panaretou, Peter Piper
Mutagenesis Leslie Barbour, Michelle Hanna, Wei Xiao
Production of heterologous proteins in yeast with the aid of the Hsp150Δ Carrier Marja Makarow
Pulsed field gel electrophoresis of budding yeast chromosomes David Lydall, Laura Maringele
Purification of yeast peroxisomes Astrid Kragt, Ben Distal
Study of transcriptional regulation using a reporter gene assay Wei Xiao, Yu Fu
Synthetic genetic array analysis in saccharomyces cerevisiae Amy Hin Yan Tong, Charles Boone
Synthetic lethal screen Leslie Barbour, Wei Xiao
Two-dimensional agarose gel analysis of DNA replication intermediates Alain T. Dandjinou
Two-dimensional gel electrophoresis of total yeast proteins Christelle Monribot-Espagne, Hélian Boucherie
Yeast fluorescence microscopy Jiří Hašek
Yeast transformed by the LiAc/SS carrier DNA/PEG method R. Daniel Gietz, Robin A. Woods
Yeast two-hybrid system screening R. Daniel Gietz
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